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|
Gases mL/culture
|
Forage to concentrate ratio
|
P-value
|
|---|
|
9:1
|
7:3
|
5:5
|
3:7
|
1:9
|
|---|
|
Control
|
Treatment
|
Control
|
Treatment
|
Control
|
Treatment
|
Control
|
Treatment
|
Control
|
Treatment
|
Additive
|
Diet
|
A × D
|
|---|
|
Cashew nut shell liquid
|
|
Total gas
|
4.00abc
|
3.58bc
|
4.84ab
|
4.27abc
|
4.87ab
|
3.32c
|
5.08a
|
3.56bc
|
4.81ab
|
3.69abc
|
<0.001
|
0.173
|
0.213
|
|
CO2
|
3.44ab
|
2.96ab
|
3.95ab
|
3.72ab
|
3.93ab
|
2.81b
|
4.08a
|
3.01ab
|
3.78ab
|
3.18ab
|
<0.001
|
0.200
|
0.349
|
|
CH4
|
0.54bc (100)
|
0.60bc (111)
|
0.88abc (100)
|
0.54c (61)
|
0.93ab (100)
|
0.50c (54)
|
0.98a (100)
|
0.53c (54)
|
1.02a (100)
|
0.50c (49)
|
<0.001
|
0.103
|
0.008
|
|
H2
|
0.01
|
0.02
|
0.01
|
0.02
|
0.01
|
0.01
|
0.01
|
0.01
|
0.01
|
0.01
|
0.744
|
0.466
|
0.428
|
|
Monensin
|
|
Total gas
|
4.62
|
4.44
|
5.75
|
4.80
|
5.47
|
4.82
|
5.17
|
4.68
|
5.31
|
4.39
|
0.009
|
0.442
|
0.742
|
|
CO2
|
3.61
|
3.30
|
4.47
|
3.71
|
4.03
|
3.69
|
3.79
|
3.66
|
3.96
|
3.50
|
0.059
|
0.535
|
0.875
|
|
CH4
|
1.13ab (100)
|
1.14ab (101)
|
1.27a (100)
|
1.08ab (85)
|
1.43a (100)
|
1.12ab (85)
|
1.36a (100)
|
1.01ab (78)
|
1.35a (100)
|
0.73b (54)
|
<0.001
|
0.295
|
0.092
|
|
H2
|
0.01
|
0.01
|
0.02
|
0.01
|
0.01
|
0.01
|
0.01
|
0.01
|
0.01
|
0.01
|
0.001
|
0.642
|
0.949
|
- Values in parenthesis are relative percentages of methane production in treatment to that in control
- A × D indicates interaction between additive and diet effects
-
a-cMeans within a row with different superscripts significantly differ (P < 0.05)